|
KMID : 1149220190270020101
|
|
Korean Journal of Oriental Medical Prescription
2019 Volume.27 No. 2 p.101 ~ p.120
|
|
|
Anti-inflammatory effect of Euonymi Lignum Suberalatum in LPS-activated Raw 264.7 cells and CA-induced paw edema rat model
|
|
Jeon Chang-Gwon
Park Sang-Mi
Park Chung-A
Byun Sung-Hee
Kim Sang-Chan
|
|
|
Abstract
|
|
|
PURPOSE : Euonymi Lignum Suberalatum (EL) is the stem fin of Euonymi alatus. In traditional Korean medicine, EL is used for treatment of uterine bleeding, metritis and static blood. Recently, many studies have reported several pharmacological effects of EL including anticancer, antimicrobial, antidiabetic activity, and anti-oxidative stress. However, the mechanisms underlying anti-inflammatory effects by the EL is not established.
METHODS : To investigate anti-inflammatory effects of Euonymi Lignum Suberalatum Water (ELWE), Raw 264.7 cells were pre-treated with 10-300 ㎍/mL of ELWE, and then exposed to 1 ㎍/mL of LPS. Levels of NO, IL-6, IL-1β and TNF-α were detected by ELISA kit. Expression of pro-inflammatory proteins were determined by immunoblot analysis. To evaluate the anti-inflammatory effect in vivo, rat paw edema volume, and expressions of COX-2 and iNOS proteins in carrageenan (CA)-induced rat paw edema model.
RESULTS : NO production activated by LPS, was decreased by 30-300 ㎍/mL of ELWE. Production of inflammatory mediators such as TNF-α, ILs, PGE2 were decreased by ELWE 100 and 300 ㎍/mL. In addition, ELWE reduced LPS-mediated iNOS and COX-2 expression. Moreover, ELWE increased I-κBα expression in cytoplasm and decreased NF-κB expression in nucleus. In vivo study, ELWE reduced the increases of paw swelling, and expression of iNOS and COX-2 proteins in paw edema induced by CA injection.
CONCLUSION : The results indicate that ELWE could inhibit the acute inflammatory response, via modulation of NF-κB activation. Furthermore, inhibition of rat paw edema induced by CA is considered as clear evidence that ELWE may be a useful source to treat acute inflammation.
|
|
|
KEYWORD
|
|
|
Euonymi Lignum Suberalatum, Anti-inflammatory effect, Cytokine, NF-κB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|